Enhancing Gene Loading of Polyamidoamine (PAMAM) dendrimer by Modification with Polyethyleneimine (PEI) as a Nanocarrier for Gene Delivery
Paper ID : 1644-ICNS
Mohammad Amiri1, Akbar Shojaei *2, Beheshteh Khodadadi3, Fatemeh Chegini4, Shohreh Mashayekhan5, Mahdi Karimi3
1INST, Sharif University of Technology
2Department of Chemical Engineering, Sharif University of Technology, P.O. Box 11155-9465, Tehran, Iran
3Iran University of Medical Sciences
4Iran University of MEdical Sciences
5Sharif University of Technology
Polyamidoamine (PAMAM) dendrimer and polyethyleneimine (PEI) are the most popular nanocarrier in gene delivery. Although biocompatibility of PAMAM is better than PEI, gene loading and toxicity of PEI is higher than PAMAM. In this study, in order to improve gene capacity of PAMAM, we synthesised a nanocarrier with PEI core with conjugated PAMAM dendrimer as shell. This dendrimer was synthesized in a two-step process. First, methyl acrylate (MA) was linked to PEI core, and then PAMAM shells were formed by attaching ethylene diamine (EDA) to MA. Gel retardation studies were carried out to find the ability of nanocarriers for DNA binding. Our data indicated that PEI/MA+EDA (PEI/PAMAM) nanostructure can have better binding, loading and condensing for plasmid DNA (pDNA). This behaviour was attributed to the addition of PEI to PAMAM. The new nanocarrier synthesized in this study exhibited higher loading up to fivefold and less toxicity compared with PAMAM, because of the presence of PEI in final structure which is partially replaced by PAMAM.
Gene delivery, Nanocarrier, Polyamidoamine, Polyethyleneimine, Copolymer
Status : Abstract Accepted (Poster Presentation)