Magnetic molecularly imprinted polymer for the selective dispersive micro solid phase extraction of phenolphthalein in urine samples and herbal slimming capsules prior to HPLC-DAD analysis
Paper ID : 1551-ICNS
Authors:
Niloofar Jalilian *1, Homeira Ebrahimzadeh1, Ali Akbar Asgharinezhad2
1Faculty of Chemistry and Petroleum Sciences, Shahid Beheshti University, G.C., Evin, Tehran, Iran
2Chemistry and Process Department, Niroo Research Institute, Tehran, Iran
Abstract:
In this study, a new magnetic MIP based on amino functionalized Fe3O4@SiO2 NPs was synthesized. The proposed MMIPs-D-µ-SPE method was effectively utilized for the selective extraction of phenolphthalein from urine samples and herbal slimming capsules prior to HPLC-DAD analysis. Different characterization methods including field emission scanning electron microscopy (FESEM), fourier transform-infrared spectroscopy (FT-IR), energy dispersive X-ray spectroscopy (EDX), thermogravimetric analysis (TGA), Xray diffraction (XRD) were applied for characterizations of synthesized MMIP. Parameters affecting the phenolphthalein extraction efficiency such as pH, amount of MMIP, sorption time, eluent type, eluent volume and elution time were investigated. The maximum sorption capacity of the MMIP and MNIP were 138.2 and 68.5 mg g−1 for phenolphthalein, respectively. Under the optimum conditions, the calibration curve was linear in the concentration range of 0.05-10000 µg L-1 and the detection limit (LOD) was found 0.01 µg L-1 (S/N = 3). The RSD values at three concentration levels (50, 250 and 500 µg L-1) ranged from 2.7% to 3.4% (intra-day, n=5) and 4.5%-5.5% (inter-day) precision for three varied days. The present study proposed promising prospects of environmentally friendly MMIP based D-µ-SPE-HPLC-DAD method for highly efficient sample pretreatment and intended analytes determination in complex matrices.
Keywords:
Magnetic molecularly imprinted polymer; dispersive micro solid phase extraction; phenolphthalein; herbal slimming capsules; urine samples.
Status : Abstract Accepted (Poster Presentation)