Mechanistic insight into Consequences of ROS Burst by Ag@ZnO NPs in Remanent Melanoma Cancer Cells
Paper ID : 1522-ICNS
Behnaz Ghaemi *
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
literature shows that cancer cell death accompanied by organelle dysfunction might be a promising approach for cancer therapy specially in remanent cancer cells. The Golgi apparatus initiates signaling pathways to mitigate stress and, if irreparable, start apoptosis. It has been shown that Golgi disassembly and fragmentation during oxidative stress act as indicator for stress-mediated cell death pathways through cell cycle arrest in the G2/M phase. The present study shows that UV-induced reactive oxygen species (ROS) generation by Ag@ZnO nanoparticles (NPs) in survived cancer cells, transform the Golgi structures from compressed perinuclear ribbons into detached vesicle-like structures distributed in the entire cytoplasm of melanoma cells. Afterward, Golgi fragmentation triggers G2 block of the cell-cycle progression, preventing cells from entering the mitosis phase and promotes autophagy. Taken together, Ag@ZnO NPs induce stress-related Golgi fragmentation and autophagy, finally leading to late apoptosis in melanoma cells. Intracellular oxidative stress generated by Ag@ZnO NPs upon UV irradiation may thus represent a targeted approach to induce cancer cell death through organelle destruction whereas fibroblast cells remained significantly unaffected.
Ag@ZnO NPs; ROS Burst; Golgi fragmentation; autophagy; Apoptosis; cell cycle arrest
Status : Abstract Accepted (Poster Presentation)