Enzyme-Modified Mesoporous Silica Nanoparticles as Antibacterial Agents in Vitro
Paper ID : 1510-ICNS
Parvaneh Esmaeilnejad-Ahranjani *1, Ayyoob Arpanaei2
1Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
2Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14965/161, Tehran, Iran
The antibacterial activity of lysozyme-immobilized mesoporous silica (MS) nanoparticles as a novel antibacterial delivery system was evaluated. Uniformly sized MS nanoparticles with mean diameter of 72±8 nm were prepared by template removing method. The prepared particles were carboxyl-functionalized (COOH-MS) and lysozyme molecules were physically (Lys-Ads-MS) and covalently (Lys-Cov-MS) attached onto the particles. The lysozyme immobilization efficiencies of Lys-Ads-MS and Lys-Cov-MS were about 80 and 76%, respectively. The minimal inhibition concentration (MIC) of the Lys-Ads-MS and Lys-Cov-MS were about 4.5 and 4.1 fold lower than the free lysozymes in vitro, respectively. The new represented antibacterial agents showed no cytotoxicity in vitro. These natural antibacterial enzyme-modified nanoparticles open up a new avenue for the design of next-generation antibacterial agents as alternatives to antibiotics.
Mesoporous silica nanoparticles; Lysozyme immobilization; Antibacterial activity.
Status : Abstract Accepted (Poster Presentation)