A novel fluorescent biosensor based on a metal organic framework nanocomposite with peroxidase mimic properties for xanthine sensing in serum samples
Paper ID : 1505-ICNS
Farzaneh Ahmadian, Hossein Abdolmohammad-Zadeh *
Analytical Spectroscopy Research Lab., Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz,53714–161, Iran
In this research, ferrite/zirconium (Zr)-based metal organic framework (MOF) nanocomposite (ferrite/UiO–66) with great peroxidise-like activity was successfully applied for selective determination of xanthine. A facile and efficient method was developed for the preparation of ferrite/UiO–66 nanocomposite with superior peroxidise-like activity. The nanocomposite was fabricated via bottom–up synthesis strategy. Detailed characterization including SEM, XRD analysis was done to characterize ferrite/UiO–66 nanocomposite. The high surface area of UiO–66 remarkably improved the catalytic activity of ferrite. The as-synthesized ferrite/UiO–66 nanocomposite possessed fluorescence property (λem= 425 nm) as well as peroxidase mimic activity. On the basis of these two properties, we introduced a biosensor for indirect xanthine detection. The fluorescence intensity had a linear relationship with the xanthine concentration in the range of 8–250 µmol L−1. The limit of detection was 2.0 µmol L−1. This work contributes to the synthesis of a new MOF-based nanocomposite and extends the application of MOF nanocomposite on luminescent sensors. The developed method was able to selectively measure xanthine in a complex media without significant interfering effects from analogue compounds.
Metal organic framework; Nanocopmosite; Luminescent sensor; Peroxidase Mimetic; Xanthine
Status : Abstract Accepted (Oral Presentation)