Effect of Curcumin-Au Nanoparticles on SK-N-SH cells: Synchrotron Radiation Based IR Microspectroscopy
Paper ID : 1475-ICNS
Authors:
Fateme Karimi Hafshejani1, Sharmin Kharrazi *2, Mohammad Hossein Ghahremani3, Ibraheem Yousef4, Imma Martinez-Rovira4
1Tehran university of medical sciences, Tehran. Iran
2Tehran university of medical sciences, Tehran, Iran
3Tehran University of Medical Sciences, Tehran, Iran
4MIRAS Beamline, ALBA Synchrotron, Barcelona, Spain
Abstract:
Oxidative stress has been proved as a contributor over 100 human diseases, either as source or outcome. Trace of oxidative stress in the etiology of several chronic and degenerative diseases suggests that antioxidant therapy represents a promising avenue for therapeutics. An important group of antioxidants is phenolic compounds. However, their extremely low water solubility, poor stability, and passive diffusion restricts their bioactivity. Curcumin as an antioxidant was used to reduce and synthesis of nanoparticles by using biological synthesis method. Curcumin coated gold nanoparticles (CUR-GNPs) were chemically synthesized and characterized using UV-vis spectrophotometry and TEM. Particle size was found to be 21.7 ± 5.7 by TEM and used in cell studies. The antioxidant activity of nanoparticles investigated on the H2O2-treated SK-N-SH cell line. For exact understanding of the effects of CUR-GNPs on DNA, proteins, and lipid of these cells, fourier transform infrared (FTIR) microspectroscopy experiments were performed at the MIRAS laboratory of the ALBA synchrotron. This includes DNA fragmentation, lipid peroxidation (changes in the CH2 and CH3 stretching modes) and alternations in the intensity and the position of the amide I and II bands.
Keywords:
CUR-GNPs; Antioxidant; SK-N-SH cell line; H2O2; Infrared Microspectroscopy
Status : Abstract Accepted (Poster Presentation)