The effect of magnetic hyperthermia on the quality of ovarian tissue and oxidative stress in a sheep model
Paper ID : 1461-ICNS
Sareh Karimi1, Nasrollah Tabatabaei *2, Alireza Ebrahimzadeh-bideskan3, Zahra Shams mofarahe1
1Dept. of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2School of Advanced Technologies in Medicine, Tehran University of Sciences, Tehran, Iran
3Department of Anatomy and cell biology, School of Medicine, Mashhad University of Medical Sciences
Background: Today, because of the elevated counts of infertility between couples, fertility preservation is recognized as a global public health problem by the WHO. One of the methods of fertility preservation is ovarian cryopreservation, however this technique risks a decrease in the quality of tissue after vitrification and thawing. In this study we examine a new method to quickly thaw the vitrified ovarian tissues.
Method: After synthesis, coat and characterization of the IONPs, ovarian tissue of sheep (as human model) was vitrified with vitrification solutions with and without IONPs. In time of warming, vitrified tissue was placed inside an alternating magnetic field. Then, penetration of IONPs was assay with Prussian blue staining and quality of tissue was assayed by H&E staining.
Result: The total duration of thawing of vitrified tissue with IONPs was less than control, and the quality of tissue was better.
Conclusion: The results demonstrate biological evidence that magnetic hyperthermia can improve the outcome of vitrified cryogenic storage of ovarian tissues.
IONP, Magnetic hyperthermia, Ovarian tissue
Status : Abstract Accepted (Poster Presentation)