Investigation of interaction of diamond nanoparticles with human chorionic gonadotropin (hCG)
Paper ID : 1455-ICNS
zeinab akbarkashani *1, Azadeh Hekmat2, Seyed Mohammad Atyabi3
2Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3Department of Nanotechnology, Pastor Institute, Tehran, Iran
Human chorionic gonadotropin (hCG) is a hormone released by the placenta and some tumors. Studies have shown that most cancerous tumors have hCG that promotes tumor growth, and research has been done to inhibit it during cancer. This study has been carried out through spectroscopic methods, changes of hCG structure before and after the interaction with the nanodiamond at 37 ° C and physiological pH (7.4). Analysis of the UV-visible spectrum indicates the exposure of tyrosine residues to the surface of hCG. This indicates that hCG is adsorbed on the nanoparticles. The fluorescence spectrum indicates a change in the tertiary structure of the protein and changing the position of Tyr residues and the exposure of Tyr residues to the surface of hCG, which might be the reason why fluorescence intensity increased and the bioactivity decreased with the increasing concentration of Nanodiamonds.
hCG, Nanodiamonds, Tumor, Interaction
Status : Abstract Accepted (Poster Presentation)