Osteogenic effect of PDA-coated 3D printed nanocomposite scaffolds
Paper ID : 1442-ICNS
Behnoush Samavati1, Elnaz Tamjid *2, Omid Cheraghi3, Khosro khajeh3
1Department of Biomaterials, Faculty of Multidisciplinary Science and Technology, Tarbiat Modares University, Tehran, 14115-175, Tehran, Iran
2Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
3Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, 14115-175, Iran
3D printed porous scaffolds are anticipated to generate biophysical and biochemical support to the surrounding cells to resemble extra cellular matrix (ECM). Thus, appropriate bone tissue engineering scaffold is intended to provide appropriate hydrophilicity, biocompatibility, and delivering diverse bioactive molecules such as growth factor and enzymes. In this study, polydopamine (PDA) was used as surface modification on PCL/TiO2 nanocomposite scaffolds fabricated by 3D-printing. The scaffolds were characterized by contact angle, compression strength, SEM, EDX, and XRD tests. In vitro cell studies were performed by MTT and ALP enzyme activity assays on mesenchymal stem cells (MSCs) seeded onto PCL, PCL/nTiO2, and PCL/nTiO2/PDA scaffolds. The results showed that the addition of TiO2 nanoparticle and dopamine surface modification increased the hydrophilicity of PCL with acceptable cell viability. ALP enzyme activity assay determined a minor change in differentiation on dopamine-coated PCL/nTiO2 scaffolds in comparison with PCL scaffolds after 3 days of cell culture. Nevertheless, a significant change was noticed after 10 days. The results have indicated that PDA-coated nanocomposite scaffolds are potentially promising for bone tissue engineering applications.
3D printing; Nanocomposite scaffold; Dopamine; Cytocompatibility; Differentiation
Status : Abstract Accepted (Poster Presentation)