Facile label free immunosensor for early diagnosis prostate cancer via ultrasensitive detection of PSA biomarker
Paper ID : 1435-ICNS
Authors:
Parnaz Assari *1, Amirabbas Rafati2
1Bu-Ali sina university
2Department of Physical Chemistry, Faculty of Chemistry, Bu-Ali Sina University, P.O.Box 65174, Hamedan, Iran
Abstract:
In this paper, a novel label free electrochemical immunosensor was introduced based on glassy carbon electrode (GCE) modified with carboxylated carbon nanotube (C-MWCNT)/ gold nanoparticles (AuNPs)/ polyaniline (PANI) for the detection of prostate specific antigen (PSA). The precise magnitude of prostate specific antigen (PSA) tumor marker in blood is vital for early cancer diagnosis and clinical management. The AuNPs were utilized as a connector for PSA antibody immobilization through NH2 groups on antibody. Investigations on modified electrode surface were performed by FT-IR spectrum and scanning electron microscope (SEM). The result of FT-IR confirmed the electrodeposition of AuNPs on the electrode surface. According to SEM image, COOH-MWCNTs/PANI/AuNPs were immobilizedAs a sensitive analytical method for the detection of PSA, differential pulse voltammetry (DPV) was employed in different ranges of antigen concentration, 33 pg.mL-1 to 1.5 ng.mL-1, from the linear relationship between antigen concentration and peak current. Additionally, the equation of the calibration plot is I(µA) = -142.52 C(ng.mL-1)+313.32 (R2=0.9902).
Keywords:
Prostate specific antigen, Immunosensor, Polyaniline, Electropolymerization
Status : Abstract Accepted (Poster Presentation)