Disposable biosensor for Detection of Staphylococcus aureus Enterotoxin B
Paper ID : 1401-ICNS
Mir-Hassan Moosavy *
Department of Food Hygiene and Aquatic Faculty of Veterinary Medicine University of Tabriz, Tabriz, Iran
SEB is a water soluble, highly stable, toxin which causes symptoms ranging from nausea through to toxic-shock-syndrome. SEB is not only an important cause of foodborne intoxication but is also a recognized Class B bioterrror agent. Therefore, there is an identified need for rapid on-site diagnostic tests that can detect toxin at levels of <1ng. Because SEB is hazardous to handle and expensive the sensor was optimized using myoglobin. Although structurally different to SEB, myoglobin has a comparable molecular weight (17kDa) and hence was used as a surrogate for the toxin. The main objective of the study was to develop disposable electrochemical biosensors based on polymer electrodes. Different concentrations of myoglobin were adsorbed on the surface of polypropylene poly-liquid electrodes and reacted with anti-myoglobin-Dynabeads. In parallel, the BSA replaced myoglobin to determine the amount of non-specific binding. The biosensor was used to measure different amounts of enterotoxin B (0.05 to 40000 ng), and the results showed that the biosensor could detect the concentration of 0.05ng of enterotoxin B.
Biosensor, Staphylococcus aureus, Enterotoxin B, Polycarbonate membrane
Status : Abstract Accepted (Poster Presentation)