Improved in Vitro Endothelialization and Degradation of Polyurethane for Bypass Graft Applications
Paper ID : 1389-ICNS
Ghazal Kianpour *1, reza bagheri2, Ali Pourjavadi3, Hossein Ghanbari4
1Institute for Nanoscience and Nanotechnology, Sharif University of Technology, Tehran, Iran.
2Polymeric Material Research Group, Department of Materials Science and Engineering, Sharif University of Technology, Tehran
3Department of Chemistry, Sharif University of Technology, Tehran, 11365-9516, Iran
4Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, TUMS, Tehran, Iran
Despite the urgent need for substitutes for blocked blood vessels with low-diameter (< 6 mm), no suitable alternatives have been developed for this that are FDA approved for clinical use, and this requires further research in this regard. In this work, the in vitro endothelial response of human umbilical vein endothelial cells (HUVEC) was investigated on a flat PCL- based polyurethane (PPU) surface vs a nanostructure in situ synthesized TiO2-polyurethane (TPU) nanocomposite surface, which have been produced as scaffolds for artificial vascular graft. The in-situ synthesis of TiO2 nanoparticles (TNPs) in PPU provided nano and micro scale cues that facilitated cellular adhesion, cell sensing, cell probing and especially cell migration, where more organized actin cytoskeletal filaments formed pseudopodia and filopodia morphologies. . The results show that the rate of degradation was approximately 2 times, and the endothelialization rate was 1.5 times for nanocomposite film vs pure polyurethane film after 8 weeks.
polyurethane; TiO2; nanocomposite; tissue engineering; bypass graft.
Status : Abstract Accepted (Poster Presentation)