Preparation of Chitosan/Quantum Dots Nanoparticles for Biomedical Applications
Paper ID : 1383-ICNS
Parinaz Sabourian *1, Seyed Sajad Ashraf1, Masoud Frounchi1, Shohreh Mashayekhan2, Sahar Kiani3
1Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran
2Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.
3Department of Brain and Cognitive Sciences, Cell Science Research Center, ROYAN Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Up to date, biocompatible polymeric nanoparticles (NPs) are an interesting area of research to carry tracking agents for imaging and targeted delivery in biomedical applications. In current study, CdSe quantum dots (QDs) were used as tracking agents. Chitosan (CS) as a biocompatible natural polymer was used to modify the surface of QDs by chemical reaction. Hyaluronic acid (HA) formed a layer on the surface of CS-QDs NPs by ionic gelation method. To determine the mean hydrodynamic diameter, size distribution and surface charge of NPs, dynamic light scattering (DLS) and zeta potential analysis were employed. Characterization of functional groups in NPs was performed using Fourier transform infrared spectroscopy (FTIR). NPs morphology and size were recorded by field-emission scanning electron microscopy (FESEM). Fluorescent microscopy showed CS-QDs/HA NPs in red fluorescence colour at ╬╗ex.= 260 nm. QDs were successfully modified with free amine groups of CS. CS-QDs/HA NPs with 122.7 nm mean size and +4.4 mV surface charges were prepared. Based on this study, CS-QDs/HA NPs are efficient carriers for bio-imaging and targeted drug delivery applications.
Chitosan; Quantum dots; Hyaluronic acid; Bio-imaging; Targeted delivery.
Status : Abstract Accepted (Poster Presentation)