Computational analysis of Lactobacillus acidophilus surface layer protein and AgNP biosynthesis using this protein and aqueous extract of dried Juglans regia green husk
Paper ID : 1349-ICNS
Authors:
Fatemeh Rahimzadeh1, Parinaz Ghadam *2, Mahboubeh Zarrabi2, Rouha Kasra Kermanshahi3
1Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
2Department of Biotechnology, Faculty of Biological Sciences, Alzahra University,Tehran, Iran
3Department of Microbiology, Faculty of Biological Sciences, Alzahra University,Tehran, Iran
Abstract:
Silver nanoparticles (AgNPs) are very interesting for researchers due to the wide range of exciting applications in various fields. Silver nanoparticles have unique properties because of their high surface area to volume ratio, but they tend to aggregate together which increases their size to micrometer scale. To overcome this challenge, biopolymers can be used to disperse and stabilize AgNPs. In this research, the surface layer proteins were extracted from Lactobacillus acidophilus ATCC4356 under stress which was applied as a template for dispersion and stabilization of AgNPs and its purity and molecular weight were analyzed by SDS-PAGE. The properties of these proteins were predicted by biology computational tools. Silver nanoparticles were produced using the surface layer proteins and aqueous extract of dried Juglans regia green husk. First, the surface layer protein was exposed to silver nitrate salt, then by adding the extract as an external reducing agent, the color change was observed over time. UV–visible spectrometry study revealed surface plasmon resonance of AgNPs around 440 nm, indicating the production of silver nanoparticles. Our introduced method does not release any hazardous side-products to the environment, so it can be suggested as a green process.
Keywords:
Silver nanoparticle; Surface layer protein; Lactobacillus acidophilus ATCC4356; Computational tools
Status : Abstract Accepted (Poster Presentation)