Novel antimicrobial hydrogel loaded with core-shell copper oxide nanoparticles hyperbranched polyglycerol /tetracycline: coated by chitosan nanoparticles
Paper ID : 1344-ICNS
Authors:
Zahra o Assadi *1, Ali o Zarrabi1, Giti o Emtiazi2, najmeh o ghorbani2
1Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan 81746‑73441, Iran
2Department of Biology, University of Isfahan, Isfahan 81746‑73441, Iran
Abstract:
PPolyvinylalcohol hydrogel was incorporated with CuO nanoparticles hyperbranched polyglycerol/tetracycline (Tet@CuO@HPG) and coated with chitosan nanoparticles (CsNp), all of them as antibacterial agents. The freeze-thaw, vacuum filtering, and dip coating methods were used for the hydrogel synthesis, incorporation of Tet@CuO@HPG and CsNp, respectively. Field emission scanning electron microscopy (FESEM), energy-dispersive X-ray spectroscopy mapping (EDAX) and atomic force microscopy (AFM) was used for the characterization of the membrane. FESEM showed the dispersity of antibacterial agents inside and outside the membrane, EDAX mapping revealed the presence of tetracycline in the membrane, and AFM proofed the CsNp coating. The release of CuO nanoparticles and tetracycline from the hydrogel accelerated the antibacterial activity while the CsNp coating designed bacterial contact inhibition. The incorporation of CuO nanoparticles into the membrane can cause the release of CuO nanoparticles, however, diminishes the antibacterial activity which showed that released tetracycline from Tet@CuO@HPG was absorbed by the CuO nanoparticles. The inhibition antibacterial assay against S.aureus and P.aeruginosa showed that the membranes without the CuO nanoparticles demonstrate the 90% antibacterial activity.
Keywords:
CuO Nanoparticle, Hyperbranched polyglycerol, PVA, Chitosan Nanoparticle, Tetracycline, Antibacterial, Hydrogel, Membrane
Status : Abstract Accepted (Oral Presentation)