Synthesis of mPEG– b- PMMA Block Copolymers via RAFT Polymerization for Delivery of Curcumin: Preparation, Characterization and in vitro evaluation
Paper ID : 1236-ICNS
Mozhgan Abedanzadeh *1, Mohsen Salmanpour1, Fatemeh Farjadian2, Ali-Mohammad Tamaddon3
1Center for nanotechnology in Drug Delivery, Shiraz University of Medical Sciences, Shiraz, Iran
2Pharmaceutical Science Research Center, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
3Department of Pharmaceutical Nanotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
Curcumin, a polyphenolic compound, is a prominent natural anticancer drug candidate which has some limiting factors such as remarkable hydrophobicity, instability and poor bioavailability, so polymeric encapsulation of curcumin was attempted to improve solubility and stability. To do so, synthesis of methoxy poly (ethylene glycol)-b-polymethyl methacrylate (mPEG-b-PMMA) amphiphilic block copolymers with variable hydrophobic lengths was performed using reversible addition-fragmentation chain transfer (RAFT) polymerization. Due to the amphiphilic nature of the copolymers, the system self-assembled in aqueous medium and formed micellar nanoparticles. Self-assembly properties of the copolymers were evaluated in terms of critical micelle concentration (CMC), particle size distribution and morphology. Curcumin was successfully loaded into the mPEG-b-PMMA micelles with a very low CMC and a curcumin solubility enhancement of about 400 times by solid dispersion method. The in-vitro cytotoxicity assay indicated that CUR-loaded mPEG-b-PMMA micelles significantly inhibited the growth of SW-48 colorectal cancer cells, A2780 ovarian carcinoma cells and HepG2 human hepatoma cells dose-dependently. Free radical scavenging activity of curcumin loaded mPEG-b-PMMA micelles demonstrated that the encapsulation of curcumin increased its stability, especially in alkaline pH. The results suggest that mPEG-b-PMMA micelles prepared at optimum hydrophobic length can be served as a promising nanocarrier in CUR delivery.
RAFT polymerization, Polymeric micelle, Curcumin, Drug delivery
Status : Abstract Accepted (Oral Presentation)