Real-Time Control on Translocation of DNA Molecules and Sequence Specific Detection via Gatable Nanopore Arrays
Paper ID : 1235-ICNS
Reza Rahighi *
SKKU Advanced Institute of Nano-Technology (SAINT), Sungkyunkwan University (SKKU), Suwon, South Korea
Target detection is of great benefit in epigenetics and cancer prognosis from early stages where both concentration and volume of sample is very low. Artificially fabricated nanopores are potentially capable of this ambition owing to their several advantages such as size tunability and superior mechanical stability. A portable device for a positive/negative test can considerably reduce total diagnosis time of diseases. However, obtaining the required selectivity that is more important than the performance sensitivity remains to be the prerequisite for clinical applications.
Peptide nucleic acid (PNA) is known for its long-term stability and selectivity in hybridizing with its complementary strand. It lacks net electrical charge, in contrast to the negative charge of DNA strands. The fact that ionic current in nanochannels (NC) is highly susceptible to its morphology and surface charge was exploited in this research. Using reactive ion etching (RIE) method, an array of NCs was made in a layered structure that acts as the gating electrodes and interior was covered with a thin dielectric material by atomic layer deposition (ALD). On-demand control on the flow of the electrolyte within each pore was achieved. Results show distinguishable difference between conductance of the as-prepared membrane prior and after incubation with the target DNA.
Bioensing, Target DNA detection, Nanopore fabrication, Translocation speed.
Status : Abstract Accepted (Oral Presentation)