Targeted delivery of doxorubicin to cancer cells by gold nanoparticles coated with AS1411 and FOXM1 aptamers
Paper ID : 1215-ICNS
Authors:
zahra khademi1, khalil Abnous *2, Seyed Mohammad Taghdisi3, Mona Alibolandi4, Mohammad Ramezani1
1Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
2Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
3Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
4Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Abstract:
This paper describes how the targeted delivery of chemotherapy drugs can improve cancer treatment. Here, aptamer-loaded Gold-chitosan nanoparticles complex was designed for targeted
delivery of doxorubicin into A549 cells and 4T1 cells as target cells. In a low pH buffer environment, the release rate of Dox was increased up to 5 times compared to Physiological Buffer. The results of flow cytometry analysis and fluorescence imaging displayed that Dox - Apt- chit-AuNPs complex was effectively uptaked into target cells, but not into CHO cells as nontarget cells. Moreover, the results of MTT assay showed that Dox - Apt- chit-AuNPs complex increased cell mortality in 4T1 and A549 cells as compared with CHO cells. Further, this complex significantly inhibited tumor growth in BALB/c mice compared with free Dox. These results suggest that targeted delivery of Dox could improve internalization into target cells and enhance the efficacy of the chemotherapeutic agents in tumor cells.
Keywords:
targeted delivery, doxorubicin, gold nanoparticles, Aptamer; Cancer treatment
Status : Abstract Accepted (Poster Presentation)