Locked nanoparticles into the polyacrylamide gel for enhancing electrophoretic separation of proteins
Paper ID : 1202-ICNS
Authors:
Sayyed Hashem Sajjadi *, Hossein Ahmadzadeh, Elaheh Kafshdar Goharshadi
Chemistry Department, Ferdowsi University of Mashhad
Abstract:
Nanoparticles (NPs) are able to improve the separation efficiency of proteins in SDS-polyacrylamide gel electrophoresis (SDS-PAGE) due to their capability in enhancing heat dissipation during electrophoresis. However, the intrinsic surface charges of NPs (at buffer pH or charge induced due to the SDS coating) make them acquire electrophoretic mobility and move in the gel. Such a movement leads to viscosity and temperature gradients in the gel and deteriorates the separation. In this work, we proposed a novel method by using surface modified silica (M-SiO2) NPs which were tethered in the gel. Modified gel was used for the electrophoretic separation of proteins. The results showed that at relatively high voltage of 250 V, although the Joule heat generated during electrophoresis disturbed the separation in the pure polyacrylamide (PA) gel, M-SiO2/PA nanocomposite gel showed better performances. In comparison with pure PA gel, the resolution increased by 32% for M-SiO2/PA in a relatively short separation time of 35 min.
Keywords:
SDS-PAGE; Joule heating; Tethered SiO2 nanoparticles; Thermal conductivity
Status : Abstract Accepted (Oral Presentation)