Thermal profile of SDS-Polyacrylamide/TiO2 composite gel during electrophoretic separation of proteins
Paper ID : 1201-ICNS
Authors:
Sayyed Hashem Sajjadi *, Elaheh Kafshdar Goharshadi, Hossein Ahmadzadeh
Chemistry Department, Ferdowsi University of Mashhad
Abstract:
Despite the fast development of novel and high-resolution electrophoresis techniques such as capillary-based methods and microfluidic devices, the slab gel electrophoresis is still a popular method for the separation of biomolecules in medicine and biology. However, producing the heat during the electrophoresis known as Joule heating inevitably leads to a rise in the gel temperature. For the first time, this work offers a whole gel temperature measurement by using a thermal camera which presents accurate temperature profiles evolving in the gel with a resolution of more than 10 pixel/mm2 and a precision of 0.1 °C. Titania, TiO2, nanoparticles (NPs) were embedded into the polyacrylamide (PA) gel to improve the electrophoretic separation of proteins. The thermal images showed that the composite PA/TiO2 gel was 2.5 °C in average cooler than pure PA gel after 15 min of electrophoresis run at 200 V. Moreover, the average number of theoretical plates over the separation of 10 protein peaks, as a criterion of separation performance, increased by about 63% at 180 V when TiO2 NPs were included into the gel.
Keywords:
Gel electrophoresis; Joule heating; TiO2 nanoparticles; Thermal conductivity
Status : Abstract Accepted (Poster Presentation)