Development of an antioxidant assay based on manganese oxide nanosheets
Paper ID : 1200-ICNS
safieh Momeni *1, Hajar Jaberie2
1Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr 75147 Iran
2Department of Biochemistry, School of Medicine, Bushehr University of Medical Sciences, Bushehr Iran
In this study, MnO2 nanosheets were for the first time employed for total antioxidant capacity (TAC) measurements. Gum Arabic, a highly branched polysaccharide, was both the reducing and templating agent for the synthesis of MnO2 nanosheets. MnO2 nanosheets are a potent oxidizing agent to oxidize tetramethylbenzidine (TMB) in physiological pH to a blue product. Due to the redox reaction between MnO2 nanosheets and antioxidants, the color intensity of the MnO2 mixture in the presence of antioxidants decreased and the absorbance difference of the resulting suspension was measured at 652 nm. The trolox equivalent antioxidant capacity (TEAC) values of several antioxidant compounds were also measured using the new assay and conventional standard CUPRAC assay (r = 0.989). Under an optimal condition, linear concentration range, detection limit, precision and accuracy of the proposed assay for the detection of various antioxidant compounds were evaluated and represented the reliability and robustness of this assay. This assay was operated in physiological pH and could be applicable in the detection of TAC in biological samples.
Manganese oxide nanosheets; antioxidant assay; TMB; colorimetric sensor; Gum Arabic.
Status : Abstract Accepted (Poster Presentation)