Electrospun multi-layer micro/nanofiberous PCL scaffold for vascular tissue engineering
Paper ID : 1171-ICNS
Shima Rashidi1, Maryam Yousefzadeh *2, Sarah Rajabi3
1Textile Engineering Department, Amirkabir University of Technology, 1591634311, Tehran, Iran
2Amirkabir University of Technology
3Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Cardiovascular disease (CVD) is the leading cause of mortality in the world. A major barrier to the development of a clinically useful small diameter vascular graft is matching the mechanical and structural properties with those of native vessels and optimizing the microenvironment to foster cell infiltration, adhesion and growth. We have developed an electrospun three-layered biodegradable scaffold based on polycaprolactone (PCL). The morphology and mechanical properties were quantified and compared with those of native vessels. Afterwards, surface modification was performed using cold plasma and an anticoagulant agent (AA) to improve an anticoagulation property.
The presence of AA on the surface of scaffolds was confirmed by EDS analysis. The scaffold showed firm integration of the three polymeric layers with no delamination. Mechanical properties were physiologically consistent, showing an elastic modulus (12.61 ± 0.55 MPa) and ultimate tensile stress (4.53 ± 0.38 MPa) comparable with native vessels in a longitude direction. Morphological and mechanical characterization showed similarities with native arterial structure, strength and elasticity. Moreover, in presence of anticoagulant agent, the time of blood clot formation was longer and at the end of each time point there were more released red blood cells.
Electrospinning; Polycaprolactone; Tubular Structures; Vascular Grafts
Status : Abstract Accepted (Poster Presentation)