A novel mesoporous sorbent for dispersive micro solid-phase extraction of busulfan from plasma samples prior to determination by HPLC- MS/MS
Paper ID : 1145-ICNS
Authors:
Fatemeh Soghra Jahed *1, Samin Hamidi2
1Department of Chemistry, Faculty of Science, Azarbayjan Shahid Madani University, Tabriz, Iran
2Food and Drug Safety Research Center, Tabriz University of Medical Science, Tabriz 51664, Iran
Abstract:
Abstract: Busulfan (Bu; 1,4-butanediol dimethanesulphonate) prescribed as a chemotherapy drug prescribed in suppressing bone marrow in cancer. Determination of busulfan concentration in patients undergoing bone marrow transplantation is necessary in order to reduce toxic effects and/or graft rejection due to unadjusted dose exposure. A new extraction method namely dispersive micro solid-phase extraction (DMSPE) based on mesoporous sorbent was used for cleaning-up the plasma samples. The melamine-formaldehyde polymer as sorbent was synthesized using a simple, one-pot polycondensation reaction by using cost-effective and abundant materials and used in drug extraction. DMSPE coupling with liquid chromatography with tandem mass spectrometry (LC-MS/MS) was implemented for the determination of the busulfan dosage in plasma samples. The linear range was found from 10-2000 ng/ml. The precision and accuracy were found better than 15% according to Food and drug administration (FDA) guideline. This method was successfully used to determine the busulfan in patients administrated busulfan as part of the preparative regimen for bone marrow transplantation.
Keywords:
Busulfan, Dispersive micro solid-phase extraction, Mesoporous sorbent
Status : Abstract Accepted (Poster Presentation)