Incredible effect of propolis-based nanoparticles on bovine insulin amyloid fibrillation
Paper ID : 1080-ICNS
Mohammad Ramezani *1, naser nikfarjam2, ebrahim Rostampour2, ali akbar meratan3
1Institute for advanced studies in basic sciences of zanjan
2Institute for Advanced Studies in Basic Sciences, Zanjan, Iran
3Inistitute for Advanced Studies in Basic Sciences, Zanjan,
An increasing body of evidence suggests that small-molecule natural products that interact with amyloid aggregates may serve as a therapeutic approach for the treatment of a range of amyloid-related diseases including neurodegenerative disorders. Propolis is a sticky substance collected by honeybees from various plants for reinforcement of the hive and banish of parasites. Propolis has been shown to possess a broad spectrum of biological activities, such as antibacterial, antiviral, anti-inflammatory, anticancer, antifungal, and antioxidative properties, and these activities are considered to be derived from the various compounds in the ethanol extract of propolis. In regard to amyloid fibrillation inhibition, some recent reports demonstrate the significant inhibitory effect of propolis-derived components on amyloid aggregation. In the present study, propolis components were first extracted by ethanol followed by pyrolysis to produce propolis-based nanoparticles. A range of techniques including Far-UV, fluorescence, and Fourier-transform infrared (FTIR) spectroscopy, and atomic force microscopy (AFM) were utilized to characterize obtained nanoparticles. Then, the efficacy of propolis-based nanoparticles to inhibit amyloid fibrillation of bovine insulin was investigated using a range of amyloid-specific techniques such as Thioflavin T and 8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence measurements, Congo red binding assay, and AFM imaging. Obtained results revealed that bovine insulin fibrillation was effectively inhibited and by propolis-based nanoparticles in a dose-dependent fashion, as in 1:10 ratio of propolis-based nanoparticles: protein we observed a nearly complete inhibition of Thioflavin T fluorescence enhancement. These results we suggest that propolis may be considered as a promising inhibitor of protein aggregation for amyloid-related diseases.
neurodegenerative disorders small-molecule amyloid aggregates Propolis
Status : Abstract Accepted (Poster Presentation)