Synthesis of Copper Ferrite Nanoparticles with Cubic Morphology And Evaluation of Its Cytotoxicity
Paper ID : 1072-ICNS
Shaghayegh Sharifi *1, Najmeh Najmoddin2, Mahmoud Ghazi Khansari3
1Research Branch Islamic Azad University
2Department of Biomedical Engineering, Islamic Azad University, Science and Research Branch, Tehran, Iran
3School of Medicine Pharmacology, Tehran University of Medical Sciences, Tehran, Iran
Today, Magnetic ferrite nanoparticles such as copper ferrite (CuFe2O4) have had a variety of engineering and medical applications.Copper ferrite (CuFe2O4) nanoparticles with cubic morphology were synthesized by hydrothermal method at 200 °C, using sodium acetate and polyethylene glycol as additives. The structure, morphology and magnetic behavior were studied using different instrumental techniques such as X-ray diffractometer (XRD), field emission scanning electron microscopy (FESEM) and vibrational sample magnetometer (VSM). The cytotoxic effects of CuFe2O4 nanoparticles in various concentrations (50, 100, 250 and 500 μg/ml) against human liver cancer cell line (HepG2) were analyzed by MTT assay after 24 h. X-ray analysis showed that the sample contained a cubic spinel structure. FESEM images showed a uniform size distribution of cubic morphology with the average size of 62 nm. Moreover, the sample showed paramagnetic behaviour. MTT assay findings suggested the increased accumulation of apoptotic bodies with the increasing concentration of CuFe2O4 nanoparticles in a dose-dependent manner.
Copper ferrite, Cubic Morphology, Hydrothermal Method, Magnetic properties, MTT assay
Status : Abstract Accepted (Poster Presentation)